Determination Clostridium perfringens

General information:

Clostridium perfringens (formerly known ca C. welchii) is o forma bacterium Gram-positive,in the form of sticks, anaerobic, spor-formation from the genus Clostridium.  C. perfringens is mereu present in class= "hps ">andi poate fi gasit ca a component normala de seection in decomposition, seminal elements, <span class= "hps ">intestinal al humans si of other vertebrates, insects, and a ground.
Referential after which is executed the analysis:

Analysis for determining the Clostridium perfringens is performed within its own laboratory according to the standard national SR EN 26461-2/2002.

Principle of the method:

Determine Clostridium perfringens is performed using the membrane filtration system.

Sample rejection cases – quantity of inappropriate sample, sample collected in inappropriate containers, samples not properly labelled, etc.

Columient – sterile container required. (purchased commercially - human pharmacies or requested from the laboratory).

The amount of sample required to perform the analysis - minimum 250ml.

Processing required after harvesting – not required.

Sample stability – freshly harvested water is stable for 3 hours at a temperature of 2...5⁰C.

The analysis method - filtrations through the

Reference values according to the Law of Drinking Water ¹⁾

Recommendations for performing the analysis/ Effects of exceeding the reference values –

Clostridas are gram-positive microorganisms, most species being considered to be saprophytic - so far about 60 species are known (of which about 30 species have produced various infections of the human host). Some of these species have the ability to cause diseases to the human host and animals. The severity of infections varies within wide limits, from simple contamination of a dirty wound to severe systemic disease.

Bacteria of the genus Clostridium can cause digestive infections, suppurative infections of deep tissue structures, infections of the skin and soft tissues, but also bacteremia. The human host can be colonized normally with clostridii, in the digestive tract or in the female genital tract (most commonly in the large intestine). Other places from which clostridas can be isolated, are represented by the oral cavity and the tegumentary surface.

In order to reduce the level of bacteria in the water, a number of methods of water chlorination can be applied depending on the severity of the infestation or UV lamps can be used.